實(shí)驗(yàn)室簡介

老年重大慢病防治中醫(yī)藥重點(diǎn)實(shí)驗(yàn)室依托西湖大學(xué)醫(yī)學(xué)院附屬杭州市第一人民醫(yī)院，由復(fù)旦大學(xué)中西醫(yī)結(jié)合研究院院長董競成教授擔(dān)任學(xué)術(shù)委員會主任。

實(shí)驗(yàn)室目前有26名研究人員，其中高級職稱11人，博士學(xué)位（博士后）20人，由副院長高越教授擔(dān)任實(shí)驗(yàn)室主任。

實(shí)驗(yàn)室面積300平方，擁有細(xì)胞實(shí)驗(yàn)平臺（二氧化碳培養(yǎng)箱、活細(xì)胞倒置熒光顯微鏡、Agilent流式細(xì)胞儀、尼康體視顯微鏡、多功能酶標(biāo)儀、生物安全柜等）；分子生物學(xué)平臺（熒光定量PCR儀、蛋白電泳系統(tǒng)、PCR儀、凝膠成像系統(tǒng)、高速冷凍離心機(jī)等）；微生物實(shí)驗(yàn)平臺（細(xì)菌搖床、細(xì)菌培養(yǎng)箱、超凈工作臺、低速離心機(jī)等）；動物實(shí)驗(yàn)平臺（線粒體功能測定儀、核磁小動物體成分分析儀、小動物能量代謝籠監(jiān)測系統(tǒng)、小動物監(jiān)測跑步機(jī)、抓力計(jì)、大小鼠移動式麻醉機(jī)、肌張力儀、小動物呼吸機(jī)、邁瑞監(jiān)護(hù)儀）等，滿足基礎(chǔ)研究需求。目前開展的小動物代謝監(jiān)測、流式細(xì)胞技術(shù)、多重核酸檢測技術(shù)，屬同行業(yè)國內(nèi)領(lǐng)先水平。

學(xué)術(shù)委員會

實(shí)驗(yàn)室主要研究方向：

1. 中西醫(yī)結(jié)合防治衰老與代謝性疾病的基礎(chǔ)與臨床研究

2. 中西醫(yī)協(xié)同防治老年腫瘤的基礎(chǔ)與臨床研究

圍繞老年醫(yī)學(xué)研究熱點(diǎn)，聚焦學(xué)科主攻方向，主要開展以下研究內(nèi)容：

（1）探索衰老與代謝性疾病（2型糖尿病、代謝相關(guān)脂肪性肝病、肌肉減少癥）的分子機(jī)制；

（2）研發(fā)肺癌、肺結(jié)節(jié)、消化道腫瘤的早期篩查方法，構(gòu)建精準(zhǔn)診斷體系；

（3）臨床與基礎(chǔ)相結(jié)合，探索中醫(yī)藥對老年重大慢病的干預(yù)療效及機(jī)制。

在研省部級以上科研項(xiàng)目：

1. 基于唾液代謝組學(xué)的肺癌早期無創(chuàng)篩查和診斷技術(shù)的建立及臨床研究（浙江省科技廳“尖兵”計(jì)劃項(xiàng)目）；

2. 骨骼肌分泌因子Musclin通過產(chǎn)熱脂肪影響全身代謝穩(wěn)態(tài)的機(jī)制研究（國家自然科學(xué)基金青年項(xiàng)目）；

3. 基于類器官高內(nèi)涵成像的益氣活血方藥效物質(zhì)及其血腦屏障保護(hù)與修復(fù)機(jī)制研究（國家自然科學(xué)基金青年項(xiàng)目）；

4. BAF60c-Myok3信號介導(dǎo)的免疫微環(huán)境重塑對肥胖和糖尿病個(gè)體骨骼肌再生的調(diào)控及機(jī)制研究（國家自然科學(xué)基金青年項(xiàng)目）；

5. SUV420H2調(diào)控肝臟糖脂代謝的機(jī)制研究（浙江省自然科學(xué)基金項(xiàng)目）；

6. 染色質(zhì)重塑因子BAF60c通過Myokine介導(dǎo)的巨噬細(xì)胞活化調(diào)控骨骼肌再生的機(jī)制研究（浙江省自然科學(xué)基金項(xiàng)目）；

7. 染色質(zhì)重塑因子BAF60b在脂肪組織產(chǎn)熱和能量代謝中的作用機(jī)制（浙江省自然科學(xué)基金項(xiàng)目）。

2022-2023發(fā)表的SCI論文:

1. Phosphorylated?YBX2?is stabilized to promote glycolysis in brown adipocytes. iScience, 2023 Oct 20.

2. Huperzine A injection ameliorates motor and cognitive abnormalities via regulating multiple pathways in a murine model of Parkinson's disease. Eur J Pharmacol. 2023 Oct 5.

3. An integrative profiling of metabolome and transcriptome in the plasma and skeletal muscle following an exercise intervention in diet-induced obese mice. Journal of Molecular Cell Biology. 2023 Aug 3.

4. The muscle-enriched myokine Musclin impairs beige fat thermogenesis and systemic energy homeostasis via Tfr1/PKA signaling in male mice. Nature communications. 2023 Jul 19.

5. The role of TSC2 in breast cancer: a literature review. Frontiers in Oncology, 2023 May 12.

6. The Combined Associations of Depression and Cognitive Impairment with Functional Disability and Mortality in Older Adults: A Population-based Study from the NHANES 2011–2014. Frontiers in Aging neuroscience, 2023, May 4.

7. Development of Nectin4/FAP-targeted CAR-T Cells Secreting IL7, CCL19 and IL12 for Malignant Solid Tumors. Frontiers in Immunology. 2022 Nov 21.

8. Mutant IDH1 attenuates hepatic lipogenesis through PTEN dependent pathway. Biochemical and Biophysical Research Communications. 2022 Nov 15.

9. Associations Between Dietary Magnesium Intake and Handgrip Strength Were Modified by Serum Vitamin D Level Among the US Older Adults. Frontiers in Nutrition, 2022, Oct 12.

10. A novel multimeric sCD19-streptavidin fusion protein for functional detection and selective expansion of CD19-targeted CAR-T cells. Cancer Med. 2022 Aug.

11. BAF60a Deficiency in Macrophage Promotes Diet-Induced Obesity and Metabolic Inflammation. Diabetes. 2022 Jul 13.

12. The Neuroprotective Effect of GM-1 Ganglioside on the Amyloid-Beta-Induced Oxidative Stress in PC-12 Cells Mediated by Nrf-2/ARE Signaling Pathway. Neurochemical Research. 2022 May 31.

13. Transcriptional control of pancreatic β-cell identity and plasticity during the pathogenesis of type 2 diabetes. J Genet Genomics. 2022 Mar 12.

14. Natural products from traditional Chinese medicine for the prevention and treatment of heart failure: progress and perspectives. Rev Cardiovasc Med. 2022, Feb 12.

15. Pharmacokinetics and Bioequivalence of a Generic Fixed-Dose Combination Tablet of Metformin Hydrochloride/Vildagliptin versus a branded product in healthy Chinese subjects under fed and fasting conditions. Clinical Pharmacology in drug development, 2022 Jan 11.

16. Dangshen Erling Decoction Ameliorates Myocardial Hypertrophy via Inhibiting Myocardial Inflammation. Front Pharmacol. 2022, Jan 3.